Regulatory

Part:BBa_K4006008:Design

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-21)


IEE5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 338
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

IEE5 should only be utilized when the upstream and downstream flanking elements of the plasmid backbone are a significant distance from the the tscA-chlN junction.

Source

10.1007/s11103-018-0776-z - Chlamydomonas reinhardtii origin

References

Macedo-Osorio, K.S., Pérez-España, V.H., Garibay-Orijel, C. et al. Intercistronic expression elements (IEE) from the chloroplast of Chlamydomonas reinhardtii can be used for the expression of foreign genes in synthetic operons. Plant Mol Biol 98, 303–317 (2018). https://doi.org/10.1007/s11103-018-0776-z